Thermo Fisher Scientific CD56/NCAM1/NKH1 Recombinant Rabbit Monoclonal Antibody (NCAM1, 2217R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 0.5–1 µg/10⁶ cells

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Formalin-fixed tissues require heating in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that functions as an adhesive molecule and is widely expressed in the nervous system in isoforms ranging from 120–180 kDa.
It is found on T cells and NK cells, involved in cell migration, axonal growth, pathfinding, and synaptic plasticity. Polysialic modification reduces CD56-mediated adhesion.
CD56 mediates homophilic and heterophilic interactions via extracellular binding to matrix components such as laminin and integrins.
It is expressed on neuroectodermal-derived cell lines, tissues, and neoplasms including retinoblastoma, medulloblastoma, astrocytomas, and neuroblastoma.
CD56 serves as a sensitive neuroendocrine marker for neuroendocrine and ovarian granulosa cell tumors.
Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.