Thermo Fisher Scientific SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), PerCP-eFluor 710, eBioscience
SSEA-4 항원을 인식하는 마우스 단클론 항체로, PerCP-eFluor 710 형광체가 결합된 제품입니다. 인간 및 다양한 종의 줄기세포 연구에 적합하며, ICC/IF 및 유세포분석(Flow Cytometry)에 사용 가능합니다. 488 nm 청색 레이저로 여기되며, 710 nm에서 방출됩니다.
- 카탈로그번호
- 46-8843-42
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), PerCP-eFluor 710, eBioscience
Thermo Fisher Scientific SSEA4 Monoclonal Antibody
Clone: eBioMC-813-70 (MC-813-70)
Conjugate: PerCP-eFluor™ 710
Brand: eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 4 publications |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Dog, Chicken, Human, Mouse, Non-human primate, Rabbit |
| Published Species | Human |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMC-813-70 (MC-813-70) |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573850 |
Available Formats
Product Specific Information
The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein.
Expression is found on human teratocarcinoma stem cells (EC), embryonic germ cells (EG), induced pluripotent stem (iPS) cells, and embryonic stem cells (ES), with a decrease upon differentiation. Expression has also been reported in bone marrow mesenchymal stem cells.
This antibody cross-reacts with mouse, rabbit, canine, chicken, and Old/New World monkeys.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested on 2102Ep cell line at 5 µL (0.06 µg) per test.
A test is defined as the amount of antibody that stains a sample in 100 µL final volume. Cell numbers may range from 10⁵ to 10⁸ cells/test.
Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
- Excitation: 488 nm (Blue Laser)
- Emission: 710 nm
Recommended filters: 685 LP dichroic mirror and 710/40 band pass filter (695/40 acceptable).
PerCP-eFluor® 710 conjugates are stable when samples are fixed overnight at 4°C in freshly prepared 2% formaldehyde.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
SSEA-4 (Stage-specific embryonic antigen 4)
A cell surface antigen expressed in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS).
In human cells, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 are down-regulated during differentiation, while SSEA-1 is upregulated.
In mouse stem cells, SSEA-1 decreases with differentiation, whereas SSEA-3 and SSEA-4 increase.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.