Thermo Fisher Scientific MUC1 Chimeric Recombinant Rabbit Monoclonal Antibody (PAM4)
MUC1 단백질의 PAM4 도메인에 특이적인 재조합 토끼 단클론 항체. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 사용 가능. HEK293 세포에서 발현된 IgG kappa 형식의 비결합 항체로, Protein A로 정제됨. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | Assay-dependent |
| Functional Assay | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PAM4 |
| Immunogen | The original antibody was raised by immunization of mice with the PAM4 antigen. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
This antibody is specific for the PAM4 domain of the human MUC1 protein.
Target Information
MUC1 (Mucin 1, Episialin, MAM-6, CA 15-3, PEM, and EMA) is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is a transmembrane glycoprotein with a large mucin-like extracellular domain that undergoes proteolysis and extensive O-linked glycosylation. MUC1 is highly polymorphic, with alleles encoding 30–90 tandem repeats, leading to significant molecular weight variation. It is abundantly expressed in over 90% of breast carcinomas and metastases. Overexpression, aberrant localization, and altered glycosylation of MUC1 are associated with carcinomas. Multiple alternatively spliced transcript variants exist, encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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