Thermo Fisher Scientific ENO1 Monoclonal Antibody (5F4)
Thermo Fisher Scientific의 ENO1 단클론 항체(5F4)는 인간 ENO1 단백질을 특이적으로 인식하며, Western blot 및 ELISA에 적합합니다. GST 태그 결합 재조합 단백질로부터 유래된 면역원으로 제작되었으며, 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5F4 |
| Immunogen | ENO1 (AAH22545, 1 a.a. – approximately 434 a.a.) full-length recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See label |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | –20°C, Avoid freeze/thaw cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein sequence:
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
Target Information
Enolase (2-phosphoglycerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. It consists of three subunits: alpha, beta, and gamma.
Non-neuronal enolase (ENO1) is an isoform composed of two alpha subunits. The ENO1 gene also encodes a shorter monomeric structural lens protein, tau-crystallin.
This multifunctional enzyme participates in:
- Glycolysis and energy metabolism
- Growth control and hypoxia tolerance
- Allergic responses and immune regulation
- Plasminogen receptor activity on cell surfaces (e.g., leukocytes, neurons)
Clinical relevance:
- Diagnostic marker for various tumors
- Marker for hypoxic brain injury and endometriosis
- Autoantigen in Hashimoto encephalopathy (HE) and Hashimoto thyroiditis (HT)
- Associated with cancer-associated retinopathy syndrome (CAR)
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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