Thermo Fisher Scientific ENO1 Monoclonal Antibody (8G8)
Thermo Fisher Scientific의 ENO1 Monoclonal Antibody (8G8)은 인간 ENO1 단백질을 인식하는 마우스 IgG1 항체로, WB, IHC, ICC, ELISA, IP 등 다양한 연구용 응용에 적합합니다. 고순도 친화 크로마토그래피로 정제되었으며, ENO1 관련 질환 연구 및 종양 마커 분석에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.3 ng/mL |
| Immunoprecipitation (IP) | 1–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8G8 |
| Immunogen | ENO1 (AAH22545, 1–434 a.a) full-length recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
Target Information
Enolase (2-phosphoglycerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits: alpha, beta, and gamma.
Non-neuronal enolase (ENO1) is an isoform composed of two alpha subunits. The ENO1 gene also encodes a shorter monomeric structural lens protein, tau-crystallin.
This multifunctional enzyme plays roles in glycolysis, growth control, hypoxia tolerance, allergic responses, and acts in the fibrinolytic system by serving as a receptor and activator of plasminogen on cell surfaces such as leukocytes and neurons.
It stimulates immunoglobulin production and is used as a diagnostic marker for various tumors and hypoxic brain injury. ENO1 is also a marker for endometriosis and associated with autoimmune diseases like Hashimoto encephalopathy and Hashimoto thyroiditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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