Thermo Fisher Scientific ENO1 Monoclonal Antibody (3C8)

Applications

Western Blot (WB)

• Tested Dilution: 1–5 µg/mL

ELISA

• Tested Dilution: 10 µg/mL

Product Specifications

Product Specific Information

Sequence of this protein is as follows:

MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK

Target Information

Enolase (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits: alpha, beta, and gamma.

Non-Neuronal Enolase (ENO1) is an isoform composed of two alpha subunits. The ENO1 gene also encodes a shorter monomeric structural lens protein, tau-crystallin.

This multifunctional enzyme plays roles in:

• Glycolysis
• Growth control
• Hypoxia tolerance
• Allergic responses

ENO1 may also act in the fibrinolytic system as a receptor and activator of plasminogen on cell surfaces such as leukocytes and neurons. It stimulates immunoglobulin production and is used as a diagnostic marker for various tumors.

In heterodimeric form (alpha/gamma), ENO1 is a marker for hypoxic brain injury post-cardiac arrest and endometriosis.
Antibodies against alpha-enolase are found in sera from patients with cancer-associated retinopathy syndrome (CAR) and identified as autoantigens in Hashimoto encephalopathy (HE), associated with Hashimoto thyroiditis (HT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.