Thermo Fisher Scientific Glucocorticoid Receptor Monoclonal Antibody (6E6)
Glucocorticoid Receptor(NR3C1)을 인식하는 마우스 단클론 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합. 인간 시료에 반응하며 약 86kDa 단백질 검출. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6E6 |
| Immunogen | Purified recombinant fragment of human NR3C1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152837 |
Product Specific Information
MA5-15801 targets NR3C1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human NR3C1 expressed in E. coli.
MA5-15801 detects NR3C1, which has a predicted molecular weight of approximately 86 kDa.
Target Information
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that functions as both a transcription factor and a regulator of other transcription factors. It is found in heteromeric cytoplasmic complexes with heat shock factors and immunophilins. Upon ligand binding, it translocates to the nucleus.
The receptor is expressed in the heart, including atria, ventricles, aorta, and other cardiac tissues.
Alternate splicing and promoter usage produce multiple transcript variants encoding identical or different isoforms.
Mutations in NR3C1 can cause glucocorticoid resistance.
The receptor binds steroids such as dexamethasone with nanomolar affinity and requires a complex of chaperone proteins for ligand activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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