Thermo Fisher Scientific PDK1 Monoclonal Antibody (4A11)
PDK1 단백질을 검출하기 위한 Thermo Fisher Scientific의 단클론 항체(4A11). Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등에 적합. 인간, 마우스, 원숭이, 랫트 시료에 반응. 44kDa PDK1 단백질 검출에 최적화됨.
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Thermo Fisher Scientific PDK1 Monoclonal Antibody (4A11)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4A11 |
| Immunogen | Purified recombinant fragment of human PDK1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152628 |
Product Specific Information
MA5-15797 targets PDK1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, Non-human primate, and Rat samples.
The immunogen is a purified recombinant fragment of human PDK1 expressed in E. coli.
MA5-15797 detects PDK1, which has a predicted molecular weight of approximately 44 kDa.
Target Information
PDK1 (3-phosphoinositide dependent kinase-1) is a serine/threonine protein kinase localized to the plasma membrane. It plays a vital role in insulin-induced glycogen synthesis, PKC activation, and activation of the AGC subfamily of protein kinases.
PDK1 is critical for cellular responses to hypoxia and supports cell proliferation under hypoxic conditions. It also protects cells against apoptosis induced by hypoxia and oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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