Thermo Fisher Scientific Phospho-HSF1 (Ser307) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of HSF1 only when phosphorylated at Ser307.

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs).
These HSEs are composed of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and located upstream of heat shock genes. Although HSEs are conserved across species, HSFs from yeast, Drosophila, and humans differ in molecular weight and show limited immunological cross-reactivity.

In humans, two HSFs (HSF1 and HSF2) have been identified, sharing 38% sequence identity and binding to the same HSEs. HSF1 responds to classical stress signals (heat, heavy metals, oxidative reagents), while HSF2 is activated during hemin-mediated differentiation of erythroleukemia cells.

HSF1 exists constitutively in the cytoplasm and nucleus as a monomer lacking DNA-binding activity. Upon stress, it becomes a nuclear-localized trimer capable of binding DNA. Phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.