Thermo Fisher Scientific Phospho-C-RAF (Tyr340) Polyclonal Antibody
C-RAF 단백질의 Tyr340 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal 항체. Western blot 및 Immunocytochemistry에 적합. Human, Mouse, Rat 반응성. 고순도 정제 및 안정적 액상 형태로 연구용에 최적화.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RAF1 (Accession P04049), corresponding to amino acid residues around phosphorylated Tyr340 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817347 |
Product Specific Information
Antibody detects endogenous levels of C-RAF only when phosphorylated at Tyr340.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates MEK1 and MEK2, which subsequently activate ERK1 and ERK2. Activated ERKs regulate gene expression involved in cell cycle, apoptosis, differentiation, and migration. Mutations in RAF1 are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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