Thermo Fisher Scientific Acid Phosphatase 1 Polyclonal Antibody
Thermo Fisher Scientific의 Acid Phosphatase 1 Polyclonal Antibody는 인간 ACP1/LMW-PTP에 특이적인 항체로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합합니다. 보존제가 없는 액상 형태이며, Protein A 및 항원 친화 크로마토그래피로 정제되었습니다.
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Thermo Fisher Scientific Acid Phosphatase 1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:4,000 |
| Immunocytochemistry (ICC/IF) | 1:300–1:10,000 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 1–4 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human ACP1/LMW-PTP Protein (Met1–His158) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787633 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human ACP1/LMW-PTP.
Target Information
Low molecular weight protein tyrosine phosphatases, including the human red cell phosphatase HCPTP, are widely expressed cytosolic proteins (~18 kDa) that exist in distinct isoforms and are highly selective for phosphotyrosine. HCPTP-A and HCPTP-B, expressed in human red cells and placenta, represent fast and slow forms of red cell acid phosphatase. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate and has a catalytic mechanism involving formation of a cystinylphosphate intermediate. The crystal structure of the bovine heart enzyme BHPTP shows a four-stranded central parallel β-sheet with flanking α-helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context (CXXXXXR).
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일명: PA5-80302_Acid_Phosphatase_1_P24666-1_Rabbit.svg, PA5-80302_Acid_Phosphatase_1_P24666-1_Rabbit_PDP.jpeg)
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