Thermo Fisher Scientific CD169 (Siglec-1) Monoclonal Antibody (7-239), PE-eFluor 610, eBioscience
인간 CD169(Siglec-1)을 인식하는 7-239 클론 단일클론 항체로, PE-eFluor 610 형광체가 결합된 제품입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 488–561 nm 레이저에서 여기됩니다. 광민감성이 있어 암조건 보관 필요.
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Thermo Fisher Scientific CD169 (Siglec-1) Monoclonal Antibody (7-239), PE-eFluor 610, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7-239 |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565/606 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574587 |
Additional Formats:
- Alexa Fluor 488
- APC
- PE
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
This 7-239 monoclonal antibody reacts with human CD169 (Siglec-1, Sialoadhesin), a type I transmembrane protein of the sialic acid binding Ig-like lectin (Siglec) family.
CD169 is expressed on macrophages and dendritic cells in peripheral blood, lymph nodes, and spleen. It binds glycoproteins and glycolipids containing N-acetylneuraminyl α2-3-galactose.
Expression can be induced on CD14+ monocytes by interferon-alpha and interferon-gamma, mediating HIV-1 infection. It also plays a role in antigen presentation to invariant NK T cells.
This antibody inhibits erythrocyte binding to CD169-expressing cells.
Reported for use in flow cytometric analysis; pre-titrated and tested on normal human peripheral blood cells.
Recommended usage: 5 µL (0.5 µg) per test, staining 10⁵–10⁸ cells in 100 µL final volume.
PE-eFluor® 610 excitation/emission: 488–561 nm / 607 nm.
Recommended filter: 610/20 band pass (equivalent to PE-Texas Red®).
Ensure instrument compatibility with this fluorochrome.
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product #00-8222)
- 1-step Fix/Lyse Solution (Product #00-5333)
Excitation: 488–561 nm
Emission: 607 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Siglecs are membrane-bound lectins of the immunoglobulin superfamily that bind sialic acid and mediate cell-cell interactions.
Family members include sialoadhesin, CD22, and CD33.
Sialoadhesin is a macrophage-specific adhesion molecule expressed on activated macrophages in chronic inflammation and tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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