Thermo Fisher Scientific CD169 (Siglec-1) Monoclonal Antibody (7-239), PE-eFluor 610, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

7-239

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

View additional formats

• Alexa Fluor 488
• APC
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574587

Product Specific Information

Description: This 7-239 monoclonal antibody reacts with human CD169, also known as Siglec-1 and Sialoadhesin. This type I transmembrane protein is a member of the sialic acid binding Ig-like lectin (Siglec) family. Expressed on macrophages and dendritic cells in peripheral blood, lymph nodes, and spleen in humans, CD169 binds glycoproteins and glycolipids containing N-acetylneuraminyl alpha 2-3-galactose. CD169 expression can also be induced on CD14+ monocytes by interferon-alpha and interferon-gamma to mediate HIV-1 infection. Finally, CD169 has also been shown to be involved in antigen presentation to invariant NK T cells.

This monoclonal antibody has been reported to inhibit erythrocyte binding to CD169-expressing cells.

Applications Reported: This 7-239 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 7-239 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.