Thermo Fisher Scientific EGF Polyclonal Antibody, PeproTech
Thermo Fisher Scientific의 EGF Polyclonal Antibody는 Rat EGF를 특이적으로 인식하는 Rabbit 유래 항체로, Western blot, ELISA, Neutralization 등에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공하며, 연구용으로 최적화된 제품.
- 카탈로그번호
- 500-P277-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific EGF Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1.0 µg/mL | – |
| ELISA | 0.5–2.0 µg/mL | – |
| Neutralization (Neu) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat EGF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930206 |
Product Specific Information
AA Sequence of recombinant protein:
MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat EGF. Anti-Rat EGF-specific antibody was purified by affinity chromatography employing an immobilized Rat EGF matrix.
Sandwich ELISA:
To detect Rat EGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required.
This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat EGF (500-P277Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat EGF.
Western Blot:
To detect Rat EGF by Western Blot analysis, this antibody can be used at a concentration of 0.5–1.0 µg/mL.
Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat EGF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
EGF (Epidermal Growth Factor) binds to the EGF receptor (EGFR), a 170 kDa protein kinase, initiating diverse cellular pathways.
Upon activation, EGFR forms homo- or heterodimers with other family members, each triggering distinct signaling cascades via recruitment of SH2-containing effector proteins.
EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands, acting as a potent mitogenic factor.
The phosphorylated receptor recruits adapter proteins like GRB2, activating downstream pathways such as RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT.
Research indicates EGF may also activate the NF-κB signaling cascade.
Defects in the EGF gene cause hypomagnesemia type 4, and dysregulation is linked to cancer progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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