Thermo Fisher Scientific PTHLH Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-PTHLH 항체로, 인간 PTHrP 검출용. Western blot과 Sandwich ELISA에 최적화. 항원 친화 크로마토그래피로 정제된 고순도 제품. 연구용으로만 사용 가능.
- 카탈로그번호
- 500-P276BT-xxxx (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific PTHLH Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 9.8 kDa Recombinant Human PTHrP (Range 37–122, UniProt ID: P12272-1) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930204 |
Product Specific Information
AA Sequence of recombinant protein:
AVSEHQLLHD KGKSIQDLRR RFFLHHLIAE IHTAEIRATS EVSPNSKPSP NTKNHPVRFG SDDEGRYLTQ ETNKVETYKE QPLKTPPreparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PTHrP. Anti-Human PTHrP-specific antibody was purified by affinity chromatography and then biotinylated.Sandwich ELISA:
To detect hPTHrP by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PTHrP (500-P276) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hPTHrP.Western Blot:
For detection of hPTHrP by Western Blot, use at 0.1–0.2 µg/mL.
With compatible secondary reagents, detection limit for Recombinant hPTHrP is 1.5–3.0 ng/lane under reducing or non-reducing conditions.Packaging:
500-P276BT-1MG will be provided as 2 × 500 µg.
Target Information
The protein encoded by the PTHLH gene is a member of the parathyroid hormone family. It regulates endochondral bone development and epithelial–mesenchymal interactions during mammary gland and tooth formation. It is also involved in lactation by regulating calcium mobilization and transfer to milk.
The receptor, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. Alternative translation initiation from non-AUG (CUG and GUG) start sites may produce nuclear forms of this hormone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: 500-P276BT-25UG_PTHLH_P12272-1_Rabbit.svg)
(이미지: 500-P276BT-25UG_PTHLH_P12272-1_Rabbit_PDP.jpeg)
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