Thermo Fisher Scientific Ly-49E/F Monoclonal Antibody (CM4), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific Ly-49E/F Monoclonal Antibody (CM4), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 2 publications

Product Specifications

Product Specific Information

This CM4 monoclonal antibody reacts with a subset of mouse Ly49 receptors, specifically Ly49E and Ly49F.
The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cells, lymphocytes that play a key role in protection from infection, malignant diseases, graft rejection, and autoimmune diseases.

Ly49E is unique from other family members in that it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains. Developmentally, Ly49E is one of the first markers detected on NK cells but expression decreases after birth to about 1% of NK cells in the adult spleen. Upon stimulation, expression can again be induced on Ly49E-negative cells.

The CM4 antibody is a useful reagent for characterization of Ly49E expression when used in combination with other Ly49 antibodies such as:

• 14B11 (Ly49C/I/F/H)
• 3D10 (Ly49C)
• YLI-90 (Ly49I)
• A1 (Ly49A)

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Use ≤ 0.5 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5. Emits at 710 nm and is excited with blue laser (488 nm).
Recommended filters:

• 685 LP dichroic mirror and 710/40 band pass filter (preferred)
• 695/40 band pass filter (alternative)

PerCP-eFluor® 710 conjugated antibodies are stable when samples are fixed with 2% formaldehyde overnight at 4°C.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cells, which play a key role in immune protection, cancer defense, graft rejection, and autoimmune regulation.
Ly49E is unique among family members due to its ITIM domain and expression across all mouse strains. It is an early NK cell marker, decreasing postnatally but inducible upon stimulation.
The CM4 antibody is valuable for Ly49E expression studies, especially when used with other Ly49-specific antibodies.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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