Thermo Fisher Scientific NSE Recombinant Rabbit Monoclonal Antibody (9B4Y4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:100-1:800

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

9B4Y4

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 47-100 of human ENO2 (NP_001966.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NSE,uniProtId:P09104-1,ncbiNodeId:9606,antigenRange:47-110,antigenLength:434,antigenImageFileName:MA5-51194_NSE_P09104-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51194_NSE_P09104-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3094176

Product Specific Information

Immunogen sequence: LELRDGDKQR YLGKGVLKAV DHINSTIAPA LISSGLSVVE QEKLDNLMLE LDGTENKSKF GANA

Target Information

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.NSE is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The alpha-subunit is expressed in most tissues, the beta-subunit only in muscle, and the gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to NSE protein are useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Further, NSE is found in elevated concentrations in plasma and certain neoplasias that include pediatric neuroblastoma and small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.