Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), Brilliant Violet 786, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532, 700
• APC, Biotin
• Brilliant UV 395, 615, 737, 805
• Brilliant Violet 421, 480, 605
• eFluor 450, FITC
• NovaFluor Blue/Red/UV/Violet/Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7
• PE-eFluor 610, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702
• Request custom conjugation available

Product Specific Information

The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185 (CXCR5/BLR1), a seven-transmembrane G protein-coupled receptor first identified in Burkitt’s lymphoma.
CD185 is expressed on B cells, CD4+ T cells, and memory (CD45RO+) T cells. It plays a critical role in lymphocyte trafficking, particularly T cell migration into B cell follicles in response to CXCL13, marking follicular helper T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.5 µg)/test
• Laser Compatibility: Violet (405 nm) laser
• Light Sensitivity: Protect from light (photo-induced oxidation)
• Fixation: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution; not compatible with methanol-based fixation
• Recommended Buffers: Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize non-specific polymer interactions

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

CXCR5 (C-X-C Chemokine Receptor Type 5, CD185), also known as Burkitt lymphoma receptor 1 (BLR1), belongs to the CXC chemokine receptor family. It is a G protein-coupled receptor expressed in lymphatic tissues and Burkitt’s lymphoma.
CXCR5 plays an essential role in B cell migration and lymphocyte trafficking. It is expressed in various tissues including lymph nodes, spleen, and tonsil. GPCRs like CXCR5 share a seven-transmembrane structure and are key targets in pharmacological research.

For Research Use Only. Not for use in diagnostic procedures.

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