Thermo Fisher Scientific BIN1 Monoclonal Antibody (12A1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,500

-

ELISA (ELISA)

1:5,000-1:50,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

12A1

Immunogen

Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with 12A exon BIN1 protein followed by hybridoma development. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BIN1,uniProtId:O00499-1,ncbiNodeId:9606,antigenRange:1-593,antigenLength:593,antigenImageFileName:209-301-E62_BIN1_O00499-1_House_mouse.svg,antigenImageFileNamePDP:209-301-E62_BIN1_O00499-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for human BIN1 protein. A BLAST analysis was used to suggest cross-reactivity with BIN1 from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.

Target Information

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.