Thermo Fisher Scientific Cytokeratin 5 Monoclonal Antibody (3E2F1)
Cytokeratin 5 단백질 검출용 Mouse monoclonal antibody. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. Human 및 Mouse 시료에 반응하며 비결합형 액상 형태로 제공. 장기 보관 시 -20°C에서 보관 권장.
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Thermo Fisher Scientific Cytokeratin 5 Monoclonal Antibody (3E2F1)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200 | 1 publication |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E2F1 |
| Immunogen | Purified recombinant fragment of CK5 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_11009375 |
Product Specific Information
MA5-15347 targets Cytokeratin 5 in indirect ELISA, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15347 immunogen is a purified recombinant fragment of CK5 expressed in E. coli.
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in molecular weight between 40–68 kDa and have an isoelectric pH between 4.9–7.8.
Human cytokeratins are numbered 1–20, and expression patterns vary depending on epithelium type and differentiation stage.
Cytokeratin subtype expression is widely used to distinguish epithelial malignancies. Cytokeratin antibodies assist in differential tumor diagnosis using immunohistochemistry and are also valuable tools in cytopathology and flow cytometry.
The composition of cytokeratin pairs varies with epithelial cell type, growth environment, and disease state. Keratins are useful markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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