Thermo Fisher Scientific GFP Monoclonal Antibody (4B10B2)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA5-15349 targets GFP in indirect ELISA, flow cytometry, immunohistochemistry, and Western blot applications, showing reactivity with Tag samples.
The immunogen is a purified recombinant fragment of GFP expressed in E. coli.
MA5-15349 detects GFP with a predicted molecular weight of approximately 27 kDa.

Target Information

The jellyfish Aequorea victoria contains Green Fluorescent Protein (GFP Tag) that emits green light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.

As a fusion tag, GFP enables localization studies, protein movement tracking, and research on subcellular compartment dynamics. GFP technology has provided significant insights into living cell physiology.

GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (395 nm) and emits green light (508 nm) without requiring additional cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications including gene expression monitoring, protein localization, protein–protein interaction studies (yeast two-hybrid), and FRET-based distance measurements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.