Thermo Fisher Scientific GFP Monoclonal Antibody (4B10B2)
GFP 단백질을 검출하기 위한 Thermo Fisher Scientific의 마우스 모노클로날 항체. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합. 단백질 태그 연구 및 형광 단백질 발현 분석에 활용 가능. 고순도 Protein G 정제 및 안정한 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | – |
| ELISA | 1:10,000 | – |
| ChIP-sequencing (ChIP-Seq) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4B10B2 |
| Immunogen | Purified recombinant fragment of GFP expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C for short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10987186 |
Product Specific Information
MA5-15349 targets GFP in indirect ELISA, flow cytometry, immunohistochemistry, and Western blot applications, showing reactivity with Tag samples.
The immunogen is a purified recombinant fragment of GFP expressed in E. coli.
MA5-15349 detects GFP with a predicted molecular weight of approximately 27 kDa.
Target Information
The jellyfish Aequorea victoria contains Green Fluorescent Protein (GFP Tag) that emits green light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP enables localization studies, protein movement tracking, and research on subcellular compartment dynamics. GFP technology has provided significant insights into living cell physiology.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (395 nm) and emits green light (508 nm) without requiring additional cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications including gene expression monitoring, protein localization, protein–protein interaction studies (yeast two-hybrid), and FRET-based distance measurements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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