Thermo Fisher Scientific His Tag Monoclonal Antibody (6G2A9), FITC, THE
His 태그 단백질 검출용 FITC 결합 단일클론 항체로, ICC/IF 및 유세포분석에 적합. 6G2A9 클론 기반 Mouse IgG1 항체. 6xHis 태그 단백질의 정제 및 검출에 활용 가능. 단백질 A로 정제된 동결건조 형태로 제공.
- 카탈로그번호
- A01620
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 0.1–2 µg/mL |
| Flow Cytometry (Flow) | 0.01–0.1 µg/mL |
| Miscellaneous PubMed (Misc) | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6G2A9 |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated (A00186-100)
- Biotin (A00613)
- HRP (A00612)
- iFluor 488 (A01800-100)
- iFluor 555 (A01801-100)
- iFluor 647 (A01802-100)
- Custom conjugation available
Product Specific Information
Reconstitute the lyophilized antibody with 100 µL of deionized water to obtain a final concentration of 1 mg/mL.
GenScript THE™ His Tag Antibody [FITC], mAb, Mouse is an FITC-conjugated THE™ His Tag Antibody purified from mouse ascites using Protein A affinity chromatography.
This product is suitable for detecting His fusion protein expression levels in various immunoassays.
- Absorption Wavelength: 492 nm
- Emission Wavelength: 518 nm
- Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Target Information
The 6xHis tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
His tags are commonly fused to recombinant proteins at the N- or C-terminus to enable purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins lacking specific antibodies.
His-tagged proteins bind strongly to Ni²⁺ through histidine residues, enabling efficient purification using Ni²⁺-NTA adsorbents.
His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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