Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin
Rat IgG 검출용 Biotin 결합 F(ab')2 Goat anti-Rat IgG (H+L) 교차흡착 2차 항체. Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능. 높은 특이성과 신호 증폭 효과 제공. 연구용으로만 사용.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536021 |
| Target | IgG |
| Cross Adsorption | Against human and mouse IgG |
| Antibody Form | F(ab`)2 Fragment |
Available Conjugate Formats
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, APC, Cyanine3, Cyanine5, DSB-X Biotin
- DyLight 488, 550, 594, 650, 680, 755, 800
- FITC, HRP, PE, Texas Red-X, TRITC
- Request custom conjugation available
Target Information
Anti-Rat secondary antibodies are affinity-purified with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies provide versatility for multiple detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification. Typically, they are produced by immunizing the host animal with pooled immunoglobulins from the target species and purified via immunoaffinity chromatography or fragmentation to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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