Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP
HRP 결합된 F(ab')2 형태의 Goat anti-Rat IgG (H+L) 고도로 교차흡착된 2차 항체로, Western blot, IHC, ELISA 등 다양한 면역실험에 적합. 높은 특이성과 낮은 비특이적 결합을 제공하며, 장기 보관 시 안정성이 우수함.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP
Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% Kathon™ CG |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536023 |
| Target | IgG |
| Cross Adsorption | Against bovine, goat, human, mouse, and rabbit IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Product has been overfilled to ensure complete recovery.
- Centrifuge to remove any particulates.
- Prepare fresh working dilution daily and discard after use.
- Shelf life: 1 year from date of receipt.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rat serum proteins, IgG from goat, hamster, human, mouse, or rabbit, and serum from bovine, goat, horse, human, mouse, or rabbit.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies provide versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
Additional Formats Available
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- TRITC
- Request custom conjugation available
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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