Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC
TRITC 결합형 염소 항 랫트 IgG (H+L) 2차 항체로, 면역형광(ICC/IF)에 적합. 고도로 교차흡수되어 사람 및 마우스 IgG에 비특이 반응 없음. F(ab`)2 절편 형태로, 높은 특이성과 낮은 배경 제공. 형광 최대치 552/578 nm.
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Applications
- Immunocytochemistry (ICC/IF): 2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation/Emission Max | 552/578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536022 |
| Target | IgG |
| Cross Adsorption | Against human and mouse IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove particulates.
- Prepare fresh working dilution daily and discard after use.
- Stable for 1 year from date of receipt.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rat serum proteins or IgG from human or mouse.
- Highly cross-adsorbed against mouse IgG.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies increase versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host with pooled immunoglobulins from the target species and are further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, APC, Biotin, Cyanine3, Cyanine5, DSB-X Biotin
- DyLight 488, 550, 594, 650, 680, 755, 800
- FITC, HRP, PE, Texas Red-X
- Custom conjugation available upon request
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