Thermo Fisher Scientific Phospho-C-RAF (Ser301) Polyclonal Antibody
C-RAF 단백질의 Ser301 인산화 형태를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot, IHC, ICC/IF에 적합하며, 인간, 마우스, 랫트 시료 반응성. 고순도 정제, 액상 형태, 연구용 전용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RAF1 (Accession P04049), corresponding to amino acid residues around phosphorylated Ser301 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2818074 |
Product Specific Information
Antibody detects endogenous levels of C-RAF only when phosphorylated at Serine 301.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates MEK1 and MEK2, which in turn activate ERK1 and ERK2. Activated ERKs regulate gene expression involved in cell division, apoptosis, differentiation, and migration. Mutations in RAF1 are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-99141_c-Raf_P04049-1_Rabbit_PDP.jpeg)
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