Thermo Fisher Scientific Phospho-AKT1/2/3 (Ser472) Polyclonal Antibody
AKT1/2/3 인산화(Ser472) 부위를 인식하는 Thermo Fisher Scientific의 폴리클로날 항체입니다. WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 사용 가능하며, Rabbit IgG 기반으로 Protein A 정제된 액상 형태입니다. 세포 생존 및 성장 신호전달 연구에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human AKT3 around the phosphorylation site of Ser472 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a serine/threonine kinase involved in regulating metabolism, cell survival, and growth. It is activated by insulin, PI3K, IGF1, and other growth factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of targets such as forkhead transcription factors and caspase-9. The AKT pathway is a major focus in cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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