Thermo Fisher Scientific Phospho-Bad (Ser118) Polyclonal Antibody
Phospho-Bad (Ser118) 항체로 인간, 마우스, 랫트 시료에 반응. Western blot, IHC, ICC, Flow cytometry, ELISA에 사용 가능. Rabbit polyclonal IgG 형식이며, Protein A 정제 및 액상 형태로 제공. 세포 사멸 조절 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:33 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human BAD around the phosphorylation site of Ser118 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by BAD is a member of the BCL-2 family, known to regulate programmed cell death (apoptosis). BAD promotes apoptosis by forming heterodimers with BCL-xL and BCL-2, counteracting their anti-apoptotic functions. Its pro-apoptotic activity is controlled via phosphorylation by kinases such as AKT and MAP kinase, and dephosphorylation by calcineurin. Alternative splicing yields two transcript variants encoding the same isoform.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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