Thermo Fisher Scientific SHH Recombinant Rabbit Monoclonal Antibody (23H15L1)

Applications

Product Specifications

Product Specific Information

This antibody is predicted to react with canine, non-human primate, mouse, rabbit, rat, and porcine based on sequence homology.

Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire

Target Information

Sonic Hedgehog (SHH) is expressed only during embryogenesis and plays a crucial role in early embryonic patterning. It acts as a key inductive signal in the development of the ventral neural tube, anterior-posterior limb axis, and ventral somites.

Among the three human proteins similar to the Drosophila Sonic Hedgehog protein, SHH is the most homologous. The protein is produced as a precursor that undergoes autocatalytic cleavage:

• The N-terminal portion is soluble and contains signaling activity.
• The C-terminal portion is involved in precursor processing and attaches a cholesterol moiety to the N-terminal product, restricting it to the cell surface.

Defects in SHH or its signaling pathway can cause holoprosencephaly (HPE), leading to forebrain malformation and facial deformities. Mutations may also contribute to VACTERL syndrome, characterized by vertebral, anal, tracheoesophageal, renal, cardiac, and limb abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지