Thermo Fisher Scientific Human IGFBP-4 His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

HEK293 cells

Tag

His-tag

Molecular weight

26 kDa

Class

Recombinant

Type

Protein

Purity

≥95% by SDS-PAGE

Endotoxin concentration

<1 EU/µg

Activity

ED50 ≤ 0.2 µg/mL; determined by the dose-dependent inhibition of MCF-7 cell proliferation in the presence of IGF2 (14 ng/mL).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Carrier-Free

Reconstitution: We recommend that the vial be briefly centrifuged to bring the contents to the bottom prior to opening. Lyophilized recombinant human IGFBP-4 should be reconstituted in sterile PBS to 0.1-1.0 mg/mL to regain full activity. These stock solutions should be apportioned into working aliquots and stored at -20°C. Further dilutions should be made in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA.

Storage: Lyophilized human IGFBP-4 should be stored at 2-8°C, preferably desiccated. Store reconstituted human IGFBP-4 at -20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.

Target Information

This gene is a member of the insulin-like growth factor binding protein family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.