
Thermo Fisher Scientific Human IGFBP-4 His-tag Recombinant Protein
인간 IGFBP-4 His-tag 재조합 단백질로 HEK293 세포에서 발현된 고순도(≥95%) 제품입니다. IGF-I 및 IGF-II 결합 연구, 세포 기능 분석, ELISA 및 Western blot 등 다양한 연구용으로 적합합니다. Carrier-free 형태로 제공되며 안정적인 보관 및 재구성이 용이합니다.
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Thermo Fisher Scientific Human IGFBP-4 His-tag Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | HEK293 cells |
| Tag | His-tag |
| Molecular Weight | 26 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥95% by SDS-PAGE |
| Endotoxin Concentration | <1 EU/µg |
| Activity | ED50 ≤ 0.2 µg/mL; determined by dose-dependent inhibition of MCF-7 cell proliferation in presence of IGF2 (14 ng/mL) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
Briefly centrifuge the vial before opening to collect contents at the bottom.
Reconstitute lyophilized recombinant human IGFBP-4 in sterile PBS to 0.1–1.0 mg/mL for full activity.
Aliquot stock solutions and store at -20°C.
Further dilutions should be made in medium or buffered solution containing carrier protein (e.g., PBS with 0.1% BSA).Storage:
Store lyophilized protein at 2–8°C, preferably desiccated.
Store reconstituted protein at -20°C (avoid frost-free freezer).
Minimize freeze-thaw cycles.
Target Information
This gene belongs to the insulin-like growth factor binding protein (IGFBP) family. It encodes a protein with both an IGFBP domain and a thyroglobulin type-I domain. The protein binds insulin-like growth factors I and II and circulates in plasma in both glycosylated and non-glycosylated forms. Binding of IGFBP-4 prolongs IGF half-life and modulates their interaction with cell surface receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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