Thermo Fisher Scientific SMAD1 Recombinant Rabbit Monoclonal Antibody (SY0254)
SMAD1 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 인간 및 마우스 반응성을 가지며, HEK293 발현 시스템에서 생산된 재조합 항체입니다. 단백질 A로 정제되어 높은 특이성과 재현성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY0254 |
| Immunogen | Synthetic peptide within human Smad1 amino acids 150–200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911567 |
Product Specific Information
- Positive Control: Hela, PANC-1, MCF-7, A431, mouse stomach tissue
- Subcellular Location: Cytoplasm, Nucleus
Target Information
The protein encoded by this gene belongs to the SMAD family, similar to the gene products of Drosophila mothers against decapentaplegic (Mad) and C. elegans Sma.
SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
SMAD1 mediates signals of bone morphogenetic proteins (BMPs), involved in cell growth, apoptosis, morphogenesis, development, and immune responses.
Upon BMP ligand binding, SMAD1 is phosphorylated by BMP receptor kinase and forms a complex with SMAD4, regulating transcription.
It is targeted by SMAD-specific E3 ubiquitin ligases (SMURF1, SMURF2) and undergoes ubiquitination and proteasome-mediated degradation.
Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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