Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Yellow 570, eBioscience

Applications and Tested Dilution

Flow Cytometry (Flow)

• Tested Dilution: 4 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue / Red / UV / Violet / Yellow series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright series
• Custom conjugation available

Product Specific Information

The OKT8 monoclonal antibody reacts with the human CD8a molecule (32–34 kDa), expressed as a heterodimer with CD8 beta chain (CD8αβ) or as a homodimer (CD8αα). It is present on thymocytes, a subset of mature T cells, and NK cells. CD8 binds to MHC class I and, through association with protein tyrosine kinase p56lck, contributes to T-cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry using human peripheral blood cells
• Recommended use: 4 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

Each NovaFluor conjugate or kit includes CellBlox Blocking Buffer.

• Add 5 µL per sample (regardless of the number of NovaFluors) when staining or preparing antibody cocktails.
• For single-color controls, add 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

• Excitation: 552 nm
• Emission: 568 nm
• Laser: 561 nm (Yellow)

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family, essential in signal transduction, T cell differentiation, and activation. It is expressed mainly on T cells as a heterodimer (CD8αβ) and also as a homodimer (CD8αα) on subsets of T cells, NK cells, monocytes, and dendritic cells.

CD8 acts as a co-receptor with TCR for MHC class I molecules and recruits Lck kinase upon ligation, leading to cytokine production and cytotoxic T lymphocyte (CTL) activation. CTLs play a crucial role in pathogen and tumor cell clearance. Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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