Thermo Fisher Scientific CD25 Monoclonal Antibody (OX39), PerCP-eFluor 710, eBioscience
Flow cytometry용 CD25 단일클론 항체(OX39)로, PerCP-eFluor 710 형광 표지되어 있음. Rat 시료에 반응하며 IL-2 수용체 알파체인 분석에 적합. 4°C 암소 보관, 동결 금지. 연구용으로만 사용 가능.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD25 Monoclonal Antibody (OX39), PerCP-eFluor 710, eBioscience
Thermo Fisher Scientific CD25 Monoclonal Antibody (OX39), PerCP-eFluor 710, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.06 µg/test
- Publications: View 6 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OX39 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10597770 |
Additional Formats
Product Specific Information
Description:
The OX39 monoclonal antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha (IL-2Rα). CD25 is expressed by early progenitors of T and B lineages and activated mature T and B lymphocytes. CD25 binds IL-2 with low affinity but forms a high-affinity receptor when associated with CD122 (IL-2Rβ) and CD132 (common γ chain). CD25 plays a role in lymphocyte differentiation and activation/proliferation.
The OX39 clone has been shown to deplete CD25 cells in vivo.
Applications Reported:
This antibody is suitable for flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of rat splenocytes. Recommended ≤ 0.06 µg/test (100 µL final volume). Optimize antibody titration for best performance.
Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited at 488 nm (blue laser). Recommended filter: 710/50 bandpass (alternative: 695/40 bandpass).
Fixation:
Samples may be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation/Emission/Laser:
- Excitation: 488 nm
- Emission: 710 nm
- Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD25 (IL2RA):
CD25 encodes the IL-2 receptor alpha chain, part of a heterotrimeric receptor complex (IL2RA, IL2RB, IL2RG) forming the high-affinity IL-2 receptor. IL2RA homodimers form low-affinity receptors. IL2 is mainly produced by mature T cells and regulates proliferation, differentiation, and apoptosis of lymphocytes. Mutations in IL2RA are associated with interleukin-2 receptor alpha deficiency and immune dysregulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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