Thermo Fisher Scientific CD38 Monoclonal Antibody (90), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD38 Monoclonal Antibody (90), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.06 µg/test
Publications: View 17 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant Violet 480
• Brilliant Violet 605
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• Request custom conjugation

Product Specific Information

The 90 monoclonal antibody reacts with the mouse CD38 molecule (~42 kDa type II transmembrane protein).
CD38 expression increases during B-cell differentiation, then decreases in germinal center B cells and plasma cells.
It is also expressed on subsets of thymocytes, mature T cells, and NK cells.
Crosslinking CD38 induces B-cell proliferation, enhanced by IL-4 and LPS.
CD38 acts as a counter-receptor for CD31 and functions as an ectoenzyme with cyclase and hydrolase activity, playing a role in lymphocyte activation and differentiation.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.06 µg/test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test. Optimize antibody titration for best results.

Fluorophore Information:
PerCP-eFluor® 710 emits at 710 nm, excited by blue laser (488 nm).
Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
Ensure instrument compatibility.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal brightness loss.

Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD38 (NAD⁺ glycohydrolase) is a type II transmembrane glycoprotein involved in activation, proliferation, and differentiation of lymphocytes, and apoptosis of progenitor cells.
Functions as a multi-catalytic ectoenzyme (ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, NAD⁺ glycohydrolase) and as a surface receptor.
Useful for lymphoma and leukemia subtyping, plasma cell detection (myeloma identification), and as a marker for activated B and T cells.
Highly expressed in pancreas, liver, kidney, malignant lymphoma, and neuroblastoma.
Associated diseases include chronic lymphocytic leukemia and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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