Thermo Fisher Scientific IgM Monoclonal Antibody (RM-7B4), PE, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 0.06 µg/test
  • View 3 publications

Product Specifications

Product Specific Information

Description:
The monoclonal antibody RM-7B4 recognizes Rat IgM antibodies and can be used as a second-step reagent in flow cytometry and microscopy. It does not recognize other rat isotypes nor cross-react with mouse IgM or any mouse isotype antibodies.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested by flow cytometric analysis of cells stained with a primary rat IgM antibody
• Recommended usage: ≤ 0.06 µg per test (100 µL final volume)
• Cell number: 10⁵–10⁸ cells/test (empirically determined)
• Titrate antibody for optimal performance

Optical Properties:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration:

• 0.2 µm post-manufacturing filtered

Target Information

Rat IgM is an immunoglobulin M isotype antibody produced by B cells in rats. It plays a crucial role in the immune system by recognizing and neutralizing antigens such as bacteria and viruses. Rat IgM is widely used in immunology, microbiology, and biotechnology research as a control reagent and diagnostic tool for detecting infectious and autoimmune diseases. Production and purification techniques include hybridoma technology, protein A/G chromatography, and affinity chromatography. Rat IgM contributes significantly to the understanding of immune mechanisms and the development of novel therapeutic approaches.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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