Thermo Fisher Scientific Rat anti-Mouse IgG2a Secondary Antibody, PE, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.06 µg/test

Product Specifications

Available Conjugate Formats

• APC
• Biotin
• eFluor 570
• FITC
• HRP
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and is suitable as a secondary reagent in flow cytometry and microscopy.
It does not recognize other mouse isotypes and shows no cross-reactivity with rat IgG2a or other rat isotypes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of cells stained with a mouse IgG2a primary antibody
• Recommended use: ≤ 0.06 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green lasers
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of target antibodies.
Secondary antibodies enhance signal amplification and versatility, supporting various detection systems (e.g., HRP, AP, fluorescence).
Typically generated by immunizing the host with pooled mouse immunoglobulins, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for high specificity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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