Thermo Fisher Scientific LAG3 Monoclonal Antibody (OTI10E7), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Flow Cytometry (Flow)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI10E7

Immunogen

Human recombinant protein fragment corresponding to amino acids 66-332 of human LAG3 produced in E.coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LAG-3 (CD223),uniProtId:P18627-1,ncbiNodeId:9606,antigenRange:66-332,antigenLength:525,antigenImageFileName:CF807146_LAG-3_CD223_P18627-1_House_mouse.svg,antigenImageFileNamePDP:CF807146_LAG-3_CD223_P18627-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.