Thermo Fisher Scientific Nogo-A Polyclonal Antibody
Nogo-A 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며 항원 친화 크로마토그래피로 정제되었습니다. 신경 재생 및 신경퇴행성 질환 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 2.5–20 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 23 amino acid peptide from near the amino terminus of human NogoA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155169 |
Product Specific Information
Despite its predicted molecular weight, NogoA typically migrates at 180 kDa in SDS-PAGE.
A suggested positive control is mouse brain tissue lysate.
PA5-20366 can be used with blocking peptide PEP-0480.
Target Information
NogoA is a member of the reticulon family of integral membrane proteins, implicated in various neurodegenerative disorders. Reticulon proteins regulate multiple cellular processes and interact with proteins such as BACE.
NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor and is highly expressed in oligodendrocytes in the CNS white matter. Blocking NogoA activity promotes axon regrowth and functional recovery in CNS lesion models.
Elevated NogoA levels have been observed in neurodegenerative diseases such as Amyotrophic Lateral Sclerosis and Multiple Sclerosis, where autoantibodies to NogoA are detected in patient samples.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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