Thermo Fisher Scientific CD163 Monoclonal Antibody (GHI/61), Biotin
인간 CD163 단백질을 인식하는 Biotin 결합 단클론 항체로, WB, IHC(F), Flow, IP 등 다양한 응용에 적합합니다. Protein G 정제, PBS/BSA 버퍼, 0.02% sodium azide 포함. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GHI/61 |
| Immunogen | Mouse monoclonal antibodies raised against human CD163 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 4–5 mg/mL BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539108 |
Additional Formats
Product Specific Information
MA5-17718 targets CD163/M130 in FACS, IHC(F), IP, and WB applications and shows reactivity with Human samples. The immunogen is mouse monoclonal antibodies raised against human CD163.
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and a member of the scavenger receptor cysteine-rich superfamily. It acts as a receptor for the hemoglobin-haptoglobin complex and protects tissues from oxidative damage caused by free hemoglobin. CD163 is expressed on human monocytes and macrophages, particularly during the late phase of inflammation. It is present on all circulating monocytes and most tissue macrophages, except certain lymphoid and epidermal cells. CD163 expression is upregulated by IL-10, IL-6, and dexamethasone, and can be shed from the cell surface by LPS or PMA stimulation. Binding to hemoglobin/haptoglobin complexes is calcium- and pH-dependent, with higher affinity for multimeric haptoglobin complexes. CD163 also induces intracellular signaling cascades involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL-6 and CSF1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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