Thermo Fisher Scientific CD62P (P-Selectin) Monoclonal Antibody (Psel.KO2.3), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 13 publications

Product Specifications

Additional Formats

• APC
• PE
• Super Bright 600
• Request custom conjugation

Product Specific Information

The Psel.KO2.3 monoclonal antibody reacts with mouse CD62P (P-selectin, GMP-140), a 140-kDa cell adhesion molecule induced on endothelial cells and platelets. During inflammation, CD62P mediates adhesion of monocytes and neutrophils to activated platelets and supports leukocyte rolling on endothelial cells. Its major ligand on neutrophils is PSGL-1. This antibody recognizes a conserved epitope across species (mouse, human, rat) and does not block cell adhesion.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on human platelets; ≤0.125 µg/test
  • Defined as the amount of antibody staining a cell sample in 100 µL final volume
  • Recommended to titrate for optimal performance
  • Effective for 10⁵–10⁸ cells/test

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm, excited with a 488 nm blue laser.
Recommended filter: 710/50 bandpass (695/40 acceptable). Ensure instrument compatibility.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)
  Storage up to 3 days at 4°C in dark with minimal signal loss. Clone-specific performance should be verified empirically.

Optical Properties:

• Excitation: 488 nm
• Emission: 710 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

P-selectin (CD62P, GMP-140, LECAM3) is a 140 kDa type I transmembrane glycoprotein involved in leukocyte-endothelial interactions. It is stored in Weibel-Palade bodies of endothelial cells and α-granules of platelets, translocating to the surface upon activation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or ADP.
P-selectin mediates adhesion between activated platelets/endothelial cells and leukocytes, contributing to inflammation, atherogenesis, thrombosis, and tissue injury. Clinically, it helps differentiate heparin-induced thrombocytopenia with or without thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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