Thermo Fisher Scientific IFN gamma Monoclonal Antibody (DB-1 (DB1)), eFluor 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 0.5 µg/test
• Publications: View 2 publications

Product Specifications

Additional Formats

• Unconjugated
• Request custom conjugation

Product Specific Information

The DB-1 monoclonal antibody reacts with rat interferon gamma (IFN-γ), a 15–20 kDa cytokine secreted by Th1 cells.

• Applications Reported: Intracellular staining followed by flow cytometric analysis.
• Applications Tested: Tested for intracellular staining and flow cytometric analysis of stimulated rat splenocytes.
• Recommended Use: ≤0.5 µg per test (in 100 µL final volume; 10⁵–10⁸ cells/test).
• Optimization: Antibody should be titrated for best performance.
• Fluorochrome: eFluor® 660 (replacement for Alexa Fluor® 647).
  • Emission: 659 nm
  • Excitation: 633 nm (Red laser)
  • Ensure instrument compatibility.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon)
A macrophage activation factor produced primarily by T-lymphocytes and NK cells in response to antigens, mitogens, and cytokines.

• Dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits
• Appears as 25, 20, and minor 15.5 kDa bands on SDS-PAGE due to differential glycosylation
• Highly species-specific biological activity (no cross-reactivity between human and mouse IFN-γ)
• Functions: antiviral activity, tumor antiproliferative activity, MHC induction, macrophage activation, enhanced B cell immunoglobulin secretion
• Acts via IFN-γ receptor I/II and JAK-STAT pathway
• Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN
• Gene mutations associated with aplastic anemia

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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