Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), eFluor 615, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), eFluor 615, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Series (Blue, Red, UV, Violet, Yellow)
• PE and PE variants (PE-Cy5, PE-Cy5.5, PE-Cy7, PE-eFluor 610)
• PerCP-Cy5.5, PerCP-eFluor 710
• Super Bright Series (436–780)
• Custom conjugation available upon request

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ or CD8αα dimers.

• CD8αβ: Found on thymocytes and mature αβ TCR T cells
• CD8αα: Found on γδ TCR T cells, intestinal intraepithelial lymphocytes, and dendritic cells

CD8 binds to MHC class I and associates with p56lck, playing a key role in T-cell development and activation.

Applications Reported: Immunohistochemistry (Frozen) and Immunocytochemistry
Applications Tested: IHC-F on mouse spleen ≤ 5 µg/mL
Filter Recommendation: Use filters capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Alexa Fluor 594 filters are acceptable.
Excitation/Emission: 595 nm / 615 nm
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin family that functions as a co-receptor for MHC class I molecules.

• Expressed on T cells as disulfide-linked heterodimers (CD8αβ) or homodimers (CD8αα)
• Involved in T cell differentiation, activation, and signal transduction
• CD8 interaction with MHC-I triggers Lck recruitment, leading to cytokine production and cytotoxic T lymphocyte (CTL) activation
• CTLs are critical for pathogen and tumor clearance
• Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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