Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), eFluor 615, eBioscience
Mouse CD8a 단백질을 인식하는 53-6.7 클론의 Rat IgG2a 단일클론 항체로 eFluor 615 형광체가 결합됨. IHC(F) 및 ICC/IF에 적합하며, 595/614 nm의 발광 특성을 가짐. T 세포 발달 및 활성화 연구용으로 사용.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), eFluor 615, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 21 |
| Immunohistochemistry (Frozen) (IHC-F) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 7 |
| Immunoprecipitation (IP) | - | 1 |
| Functional Assay | - | 2 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 615 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | eFluor™ 615 |
| Excitation / Emission Max | 595 / 614 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10805750 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV Series (395–805)
- Brilliant Violet Series (421–786)
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Series (Blue, Red, UV, Violet, Yellow)
- PE and PE variants (PE-Cy5, PE-Cy5.5, PE-Cy7, PE-eFluor 610)
- PerCP-Cy5.5, PerCP-eFluor 710
- Super Bright Series (436–780)
- Custom conjugation available upon request
Product Specific Information
The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ or CD8αα dimers.
- CD8αβ: Found on thymocytes and mature αβ TCR T cells
- CD8αα: Found on γδ TCR T cells, intestinal intraepithelial lymphocytes, and dendritic cells
CD8 binds to MHC class I and associates with p56lck, playing a key role in T-cell development and activation.
Applications Reported: Immunohistochemistry (Frozen) and Immunocytochemistry
Applications Tested: IHC-F on mouse spleen ≤ 5 µg/mL
Filter Recommendation: Use filters capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Alexa Fluor 594 filters are acceptable.
Excitation/Emission: 595 nm / 615 nm
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin family that functions as a co-receptor for MHC class I molecules.
- Expressed on T cells as disulfide-linked heterodimers (CD8αβ) or homodimers (CD8αα)
- Involved in T cell differentiation, activation, and signal transduction
- CD8 interaction with MHC-I triggers Lck recruitment, leading to cytokine production and cytotoxic T lymphocyte (CTL) activation
- CTLs are critical for pathogen and tumor clearance
- Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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