Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), eFluor 615, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

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Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

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Product Specifications

Published species

Not Applicable

Host/Isotype

Mouse / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

HI98

Conjugate

eFluor™ 615 eFluor™ 615 eFluor™ 615

View additional formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

595/614 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10804879

Product Specific Information

Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.

Applications Reported: This HI98 antibody has been reported for use in immunohistochemical staing of frozen and FFPE tissue sections (IHC-F, IHC-P), and immunocytochemical staining.

Applications Tested: This HI98 antibody has been tested by immunohistochemical staining of formalin-fixed paraffin embedded human human kidney cortex (IHC-P) using high pH antigen retrieval at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin`s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.