Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), eFluor 615, eBioscience
인간 CD15(Lewis X)를 인식하는 HI98 단클론 항체로, eFluor 615 형광 표지. IHC-F, IHC-P, ICC/IF에 적합하며, 595/614 nm에서 여기/방출. 0.2 mg/mL 액상 형태로 4°C 암소 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 5 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC-F) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI98 |
| Conjugate | eFluor™ 615 |
| Excitation / Emission Max | 595 / 614 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10804879 |
Additional Formats:
- Unconjugated
- APC
- Biotin
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
Description:
The HI98 monoclonal antibody reacts with human CD15 (Lewis X), a 3-fucosyl-N-acetyllactosamine carbohydrate moiety expressed by granulocytes. Monocytes show variable expression, while lymphocytes are negative.
Applications Reported:
Suitable for immunohistochemical staining of frozen and FFPE tissue sections (IHC-F, IHC-P) and immunocytochemical staining.
Applications Tested:
Tested in IHC-P using formalin-fixed paraffin-embedded human kidney cortex with high pH antigen retrieval at ≤5 µg/mL. Optimal performance should be determined by titration. Not validated for flow cytometry.
Filter Recommendation:
For eFluor® 615 conjugates, use filters capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Alexa Fluor® 594 filter is acceptable.
Excitation / Emission:
595 nm / 615 nm
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD15 (Lewis X, Le(x); SSEA-1):
A trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cells such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa, nervous tissue, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X.
Cells with high Le(x) expression exhibit calcium-dependent self-aggregation, relevant in embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands.
CD15 expression is widely used as a diagnostic marker in Hodgkin’s disease.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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