Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), eFluor 615, eBioscience
마우스 CD11c를 인식하는 N418 단클론 항체로 eFluor 615 형광으로 표지됨. 동결 절편 IHC 및 세포면역형광에 적합. 595/614 nm 파장 특성, 4°C 암소 보관 권장. 고순도 친화성 정제 항체로 연구용에만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 20 publications |
| Immunohistochemistry (Frozen) (IHC-F) | 5 µg/mL | 4 publications |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 5 publications |
| Flow Cytometry (Flow) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 615 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | eFluor™ 615 |
| Excitation / Emission Max | 595 / 614 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10853016 |
Additional Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395~805, Brilliant Violet 421~711, eFluor 450/506/660, FITC, Functional Grade, NovaFluor Blue/Yellow series, PE, PE-Cyanine5~7, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright 436~780.
Request custom conjugation
Product Specific Information
The N418 monoclonal antibody reacts with mouse CD11c (integrin alpha X), which associates with beta 2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, intestinal intraepithelial lymphocytes, and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of the mouse spleen and precipitates a 150, 90 kDa heterodimer.
- Applications Reported: Immunohistochemical staining of frozen tissue sections and immunocytochemistry
- Applications Tested: IHC-F on mouse spleen ≤ 5 µg/mL (optimize for best results)
- Not validated for flow cytometry
Filter Recommendation:
Use a filter capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Standard Alexa Fluor® 594 filter is acceptable.
Excitation: 595 nm
Emission: 615 nm
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD11 and CD18 form a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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