Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), eFluor 615, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395~805, Brilliant Violet 421~711, eFluor 450/506/660, FITC, Functional Grade, NovaFluor Blue/Yellow series, PE, PE-Cyanine5~7, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright 436~780.
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Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c (integrin alpha X), which associates with beta 2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, intestinal intraepithelial lymphocytes, and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of the mouse spleen and precipitates a 150, 90 kDa heterodimer.

• Applications Reported: Immunohistochemical staining of frozen tissue sections and immunocytochemistry
• Applications Tested: IHC-F on mouse spleen ≤ 5 µg/mL (optimize for best results)
• Not validated for flow cytometry

Filter Recommendation:
Use a filter capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Standard Alexa Fluor® 594 filter is acceptable.
Excitation: 595 nm
Emission: 615 nm
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11 and CD18 form a heterodimer adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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