Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), eFluor 615, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 647, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395~805, Brilliant Violet 421~786, eFluor 450/506/570, FITC, NovaFluor Blue/Yellow series, Pacific Orange, PE, PE-Cyanine5/5.5/7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436~780.
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Product Specific Information

Description:
The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells and plays an important role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported:
Reported for use in immunocytochemical and immunohistochemical staining of frozen tissue sections (IHC-F).

Applications Tested:
Tested by immunohistochemistry on frozen mouse spleen (IHC-F) at ≤1 µg/mL. Recommended to titrate for optimal performance. Not validated for flow cytometry.

Filter Recommendation:
Use a filter capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation / Emission: 595 nm / 615 nm
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD4 antigen is involved in recognition of MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed in T helper cells and may also be present in monocytes, macrophages, and dendritic cells. CD4 expression is observed in thymus, lymph nodes, tonsils, spleen, and certain regions of brain and gut. CD4 functions in early T-cell activation through association with the T-cell receptor complex and kinase Lck, and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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