Thermo Fisher Scientific Phospho-ASK1 (Ser83) Polyclonal Antibody
인간 ASK1 단백질의 Ser83 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. Protein A로 정제된 액상 형태로 제공되며, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human ASK1 around the phosphorylation site of Ser83 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase abundantly expressed in human heart and pancreas. It is activated by various extracellular stimuli, including cytokines, growth factors, and environmental stresses. ASK1, also known as MAPKKK5, activates MKK4 and MKK6, which subsequently activate JNK and p38 MAP kinases. ASK1 is activated by TNFR and Fas through interactions with TRAF family members and Daxx. Overexpression of ASK1 induces apoptosis, while inactive ASK1 inhibits TNF-α-induced apoptosis. ASK1 is expressed in a variety of human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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