Thermo Fisher Scientific Phospho-MEK5 (Ser129) Polyclonal Antibody
Phospho-MEK5 (Ser129)에 특이적인 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, ELISA 등에 사용 가능. Human MEK5 인산화 부위(Ser129) 유래 항원으로 제작. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human MEK5 around the phosphorylation site of Ser129 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MAP2K5 (MEK5) serine/threonine kinase, also known as mitogen-activated protein kinase 5, is a dual specificity protein kinase belonging to the MAP kinase family. It specifically interacts with and activates MAPK7/ERK5. MAP2K5 can be phosphorylated and activated by MAP3K3/MEKK3 and atypical protein kinase C isoforms (aPKCs). This signaling cascade is involved in growth factor–stimulated cell proliferation and muscle cell differentiation. The rs3743354 polymorphism in the MAP2K5 promoter may be associated with colorectal cancer risk.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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