Thermo Fisher Scientific MCL1 Monoclonal Antibody (OTI10C5), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and plays a critical role in cell differentiation and survival.

• Isoform 1 inhibits apoptosis, while isoform 2 promotes it.
• The C-terminal region shares homology with bcl-2.
• Expression increases upon exposure to DNA damaging agents such as ionizing or ultraviolet radiation.
• MCL1 localizes mainly to mitochondria and promotes cell viability under apoptotic conditions.
• It may interact with pro-apoptotic proteins (e.g., Bim, Bak) to inhibit apoptosis.
• MCL1 is essential for embryogenesis and lymphocyte development and exists in at least two isoforms: MCL1L and MCL1S.
• Overexpression of MCL1S promotes cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.