Thermo Fisher Scientific AIF Polyclonal Antibody
AIF 단백질을 인식하는 Goat 폴리클로날 항체로, WB, IHC, ICC/IF에 적용 가능. 인간, 생쥐, 돼지, 랫트 반응성. 액상 형태, 0.5 mg/mL 농도, TBS 버퍼에 0.5% BSA 포함. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.01–0.1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence C-NEVAKLFNIHED |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2933861 |
Product Specific Information
This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1), and isoform 4 (NP_001124318.1).
Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
Apoptosis Inducing Factor (AIF) is involved in chromatin condensation and DNA fragmentation during apoptosis. It localizes in mitochondria and translocates to the nucleus upon apoptotic induction, promoting the release of apoptogenic proteins such as cytochrome c and caspase-9.
AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, leading to severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants, and a related pseudogene exists on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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