Thermo Fisher Scientific MYLK3 Polyclonal Antibody, FITC
MYLK3 단백질을 인식하는 FITC 결합 폴리클로날 항체로, Western blot, ELISA, IP 등 다양한 실험에 사용 가능. Rabbit IgG 유래 항체이며, Human, Mouse, Rat에 반응. 액상 형태로 -20°C 암소 보관. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA (ELISA) | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 10–60 of human Myosin light chain kinase 3 (MYLK3) protein |
| Conjugate | FITC (Fluorescein Isothiocyanate) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MLCK (MLCK2), also known as MYLK3, is a myosin regulatory light chain kinase that facilitates muscle contraction. As a member of the Ser/Thr protein kinase family, MLCK phosphorylates a specific serine in the N-terminus of myosin light chains (MLC), promoting interaction with actin filaments. It plays a central role in vascular permeability and tissue edema formation. In the nervous system, MLCK regulates astrocytic process growth and transmitter release at synapses, and participates in fibroblast apoptosis signaling. Both smooth muscle and non-muscle isoforms are widely expressed, with non-muscle isoform 2 being predominant. The Telokin isoform binds calmodulin and is found in various tissues. MLCK activity is likely down-regulated by phosphorylation and the protein includes one fibronectin type III domain and nine immunoglobulin-like C2-type domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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