Thermo Fisher Scientific IL-5 Monoclonal Antibody (TRFK4), Biotin, eBioscience
마우스 IL-5를 인식하는 단클론 항체로 ELISA 검출용으로 적합. Biotin으로 표지되어 있으며, Rat IgG2a 아이소타입. 0.5 mg/mL 농도의 액상 제품으로 4°C 암소 보관. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공.
- 카탈로그번호
- 13-7051-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-5 Monoclonal Antibody (TRFK4), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| ELISA | - |
| Immunostaining (IS) | 0.5–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TRFK4 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466906 |
Additional Formats
Product Specific Information
The TRFK4 antibody reacts with mouse interleukin-5 (IL-5), also known as BCDF-alpha and eosinophil differentiation factor (EDF). This 15–20 kDa cytokine is secreted by Th2 cells and exists as a homodimer.
Applications Reported:
TRFK4 has been reported for use in detection of mouse IL-5 by ELISA.
Applications Tested:
The TRFK4 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-5 in combination with the purified TRFK5 (14-7052) antibody for capture and recombinant mouse IL-5 (14-8051) as the standard.
A suitable range of concentrations for ELISA detection is 0.5–2 µg/mL.
A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL–8 pg/mL should be included in each ELISA plate.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. It is a main regulator of eosinopoiesis, eosinophil maturation, and activation. Elevated IL-5 production is associated with asthma and hypereosinophilic syndromes.
The IL-5 receptor is a heterodimer whose beta subunit is shared with receptors for IL-3 and CSF2 (GM-CSF). IL-5, IL-4, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5, regulated by long-range elements over 120 kb on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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